Chalmers iGEM project 2024
| Reference number | |
| Coordinator | Chalmers Tekniska Högskola AB |
| Funding from Vinnova | SEK 244 330 |
| Project duration | June 2024 - October 2024 |
| Status | Ongoing |
| Venture | Working methods |
| Call | Everyday innovators in biotech and synthetic biology |
Purpose and goal
The purpose of this project is to target the global issue of increasing antimicrobial resistance by developing a silencing system that can be used with various delivery systems to achieve more effective treatment of bacterial infections. The goal is to construct several plasmid-based systems that can target and silence the expression of antibiotic resistance genes in different ways. The different systems that will be constructed are based upon CRISPR/Cas9, CRISPR/Cas13, sRNA and ribozymes.
Expected effects and result
The expected results include successfully constructing and verifying the efficiency of the silencing systems through transformation of antibiotic resistant bacteria and then adding antibiotics. Additionally, different delivery systems will be explored for future clinical application. The results will contribute to future research in the field of antibiotic resistance. By spreading information about our project and the current situation, we will raise awareness regarding the issue.
Planned approach and implementation
DNA constructs for the different silencing systems will be designed, synthesized and transferred to two model bacteria: Escherichia coli and Bacillus subtilis. Their efficiency will be assessed. Delivery systems will be examined and eventually implemented experimentally. Additionally, we will engage locally and internationally by visiting schools and other organisations, as well as participating in conferences with other iGEM teams.